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KMID : 0861020100250030019
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Korea Journal of Herbology
2010 Volume.25 No. 3 p.19 ~ p.25
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Anti-inflammatory effect of SD-01 in RAW 264.7 cells
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Park Sun-Dong
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Abstract
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Objective : The aim of this study was to investigate anti-inflammatory activity of SD-01 methanol extract in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages.
Methods : Cytotoxic activity of SD-01 methanol extract on RAW 264.7 cells was measured using 5-(3-caroboxymeth-oxyphenyl)-2H-tetra-zolium inner salt (MTS) assay. The nitric oxide (NO) production was measured by Griess reagent system. And proinflammatory cytokines and were measured by ELISA method. The levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), -B-alpha and nuclear NF- B p65 expression were detected by western blot.
Results : Our results indicated that methanol extract of SD-01 significantly inhibited the LPS-induced NO, production and iNOS, COX-2 expression accompanied by an attenuation of TNF-, IL-, IL-6 and MCP-1 production in RAW 264.7 cells. Moreover, methanol extract of SD-01 treatment also blocked LPS-induced NF-kB activation.
Conclusion : These findings indicate that methanol extract of SD-01 inhibits the production of pro-inflammatory mediators and cytokines via suppression of NF- B activation. Take together, these results indicate that methanol extract of SD-01 has the potential for use as an agent of anti-chronic inflammatory diseases.
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KEYWORD
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SD-01, oxidative stress, inflammation, macrophage
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